漆黃素固体脂质纳米粒的制备及体外释放评价
Preparation and in vivo release of fisetin solid lipid nanoparticles
王浩 1孟梦 1谢芹芹 1何宁 1王丹 2徐维平3
作者信息
- 1. 安徽中医药大学药学院,合肥 230012
- 2. 中国科学技术大学,合肥 230026
- 3. 安徽中医药大学药学院,合肥 230012;中国科学技术大学,合肥 230026;安徽省老年医学研究所,中国科学技术大学附属第一医院老年医学科(安徽省立医院),合肥 230001;中国科学技术大学附属第一医院药剂科(安徽省立医院),合肥 230001
- 折叠
摘要
目的 制备漆黄素固体脂质纳米粒(FIT-SLN),对其进行质量评价并考察其体外释放作用.方法 采用乳化蒸发-低温固化法制备FIT-SLN,利用Box-Behnken设计试验优化处方工艺,并制成冻干粉末.考察其外观、粒径、Zeta电位,并通过差示量热扫描仪判断药物在FIT-SLN冻干粉中的存在状态,透析袋法评价制剂在不同介质中的体外释放行为.结果 FIT-SLN的最佳制备工艺:漆黄素用量3 mg、吐温80用量115μL、双硬脂酸甘油酯(Precirol ATO 5)用量30 mg、卵磷脂用量30 mg.所得FIT-SLN的粒径、Zeta电位、包封率分别为(124.53±2.00)nm、(-21.33±1.69)mV、77.89％,体外释放规律与一级动力学方程相符.结论 成功制备粒径小、包封率高的FIT-SLN,其具有一定的缓释能力.
Abstract
Objective To prepare fisetin solid lipid nanoparticles(FIT-SLN),evaluate their quality and determine its in vitro release.Methods FIT-SLNs were prepared by emulsion evaporation-cryogenic curing method,and the prescription process was optimized with Box-Behnken design experiment and made into lyophilized powder.The appearance,particle size,and Zeta potential were examined,and the presence of the drug in the lyophilized powder of FIT-SLN was determined by differential scanning calorimetry.The in vitro release behavior of the preparation was evaluated by the dialysis bag method.Results The optimal preparation of FIT-SLN was as follows:3 mg fisetin,115 μL Tween 80,30 mg Precirol ATO 5,and 30 mg lecithin.The particle size,Zeta-potential,and encapsulation rate of FIT-SLN were(124.53±2.00)nm,(-21.33±1.69)mV,and 77.89％,respectively.The in vitro release was in accordance with the first-order kinetics equation.Conclusion FIT-SLN with small particle size and high encapsulation rate are successfully prepared with slowly release.
关键词
漆黄素/固体脂质纳米粒/Box-Behnken设计/体外释放Key words
fisetin/solid lipid nanoparticle/Box-Behnken design/in vitro release引用本文复制引用
出版年
2024
NETL
NSTL
万方数据