Preparation and efficacy of Suting Xuanfei granules
Objective To optimize the extraction and molding process of Suting Xuanfei granules,and determine its effect on the tracheal smooth muscle.Methods L9(34)orthogonal design experiment method was used,the content of rosmarinic acid and baicalin,and the extraction rate were used as indicators of the effect of extraction times,extraction time,and water volumn on the extraction of Suting Xuanfei granules,to optimize the optimal extraction process of Suting Xuanfei granules.The granulation condition,molding rate,moisture absorption rate,angle of repose,and solubility were used as the evaluation indicators,to determine the factors such as type of excipients and ratio of drugs to excipients in the optimal formulation molding process.The isolated rat trachea ring was prepared and soaked in K-H solution.The tension changes were measured with the BL-420S biological function system.The effects of Suting Xuanfei granules on the tension of the normal isolated rat trachea and the isolated rat trachea caused by histamine phosphate(His)and acetylcholine(ACh)were observed.The spasmolysis rate was calculated.Results The optimal extraction for Suting Xuanfei granules was as follows:adding 10 times of water,soaking for 30 min,and two extractions,each time 60 min.The average score of the three sets of orthogonal validation experiments was 92.07,and the RSD was 0.51％.The optimal molding process was as follows:the extract,dextrin,and starch were mixed at 3∶2.5∶4 to create a soft material.The mixture was then passed through a 10-mesh sieve to obtain granules.Suting Xuanfei granules showed a relaxing effect on the normally isolated tracheal smooth muscle in rats and also certain spasmolytic effect on ACh and His induced contraction of isolated tracheal smooth muscle in rats.Conclusion A stable and feasible preparation process route of Suting Xuanfei granules has been established,laying a foundation for subsequent research and development,production,and quality control.Suting Xuanfei granules has a relaxing effect on isolated tracheal smooth muscle,which may be related to antihistamine activity and the inhibition of cholinergic receptors.
Suting Xuanfei granulerosmarinus acidbaicalinpreparation processorthogonal optimizationefficacy study
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