Formulation optimization and in vitro release of ursolic acid microspheres prepared by SPG membrane emulsification
Objective To prepare sustained-release microspheres containing ursolic acid and to determine their particle size,PDI,encapsulation efficiency,drug loading capacity,and in vitro release.Methods Ursolic acid microspheres were prepared as the model drug and polylactic acid hydroxyacetic acid polymer as the carrier material,with SPG membrane emulsification,and the formulation was optimized with a single factor method.The particle size and PDI were measured with a nanoparticle size analyzer,and the morphology under an optical microscope observed.The in vitro release rate of ursolic acid microspheres was determined by UV visible spectrophotometry.Results The particle size of ursolic acid microspheres was(1.09±0.03)μm,PDI was(0.363±0.006),entrapment efficiency was(87.19±4.71)％,drug loading was(11.37±0.64)％,and the cumulative release rate in vitro was(80.53±2.82)％after 120 h.Conclusion Ursolic acid microspheres have shape round,uniform size,high entrapment efficiency and drug loading,and are reproducible.
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