Mechanism of antidepressant effect of Gastrodia elata Bl.based on network pharmacology and in vitro experiments
Objective To predict and verify the anti-depression mechanism of Gastrodia elata Bl.based on network pharmacology,molecular docking and in vitro experiments.Methods The key components and potential targets of Gastrodia elata Bl.were predicted by network pharmacology,and then molecular docking was used for the preliminary verification.The efficacy and targets of gastrodia(GAS)were verified by classical in vitro depression models.Results Network pharmacological analysis showed that 101 active ingredients and 549 key targets were collected,and 10 core targets were further obtained after the integration,including serine/thronine protein kinase 1(AKT1),tumor necrosis factor(TNF),interleukin 6(IL6),brain-derived neurotrophic factor(BDNF),etc.The key components of Gastrodia elata Bl.(GAS,4-hydroxybenzyl alcohol,and vanillin)had good binding force with these core targets.In addition,the key pathways of the antidepression effect of Gastrodia elata Bl.mainly concentrated in phosphoinositol 3-kinase/protein kinase B(PI3K-AKT),cyclic adenosine phosphate(cAMP),and hypoxic-inducing-factor-1(HIF-1).The in vitro experiments showed that GAS significantly promoted the survival of PC 12 cells and increased the expression level of BDNF and p-AKT/AKT protein,but inhibited the expression of pro-inflammation cytokines IL-6 and TNF-a.Conclusion Gastrodia elata Bl.can intervene depression via multi-components,multi-targets and multi-pathways.
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