Toxicity of repeated dose of Flemingia macrophylla(Willd.)Prain and Flemingia philippinensis Merr.et Rolfe in rats
Objective To observe the toxic reactions and differences in SD rats after oral administration of different doses of Flemingia macrophylla(Willd.)Prain and Flemingia philippinensis Merr.et Rolfe extract for 3 months,and to provide reference for clinical medication.Methods Totally 150 SD rats(75 males and 75 females)were randomly divided into 5 groups based on gender and body weight:a control group,a low-and a high-dose group of Flemingia macrophylla(Willd.)Prain(7.6 and 61.0 g crude drug·kg-1),and a low-and a high-dose group of Flemingia philippinensis Merr.et Rolfe(6.9 and 60.6 g crude drug·kg-1),with 30 rats in each group.Rats in each treatment group were given 10 mL·kg-1 orally once daily for 90 consecutive days.The body weight,food intake,hematology,blood biochemistry,blood coagulation,urine and histopathology were examined at the end of the administration and the end of the recovery.Results Compared with the control group in the same period,the body weight,food intake,hematology,blood biochemistry,blood coagulation,urine and histopathology did not show any toxicity related to drugs.Under the conditions of this experiment,SD rats were orally administered Flemingia macrophylla(Willd.)Prain and Flemingia philippinensis Merr.et Rolfe for 3 months,and the no observed adverse effect level(NOAEL)of Flemingia macrophylla(Willd.)Prain was 61.0 g crude drug·kg-1(equivalent to 22.6～67.8 times that of adult dose).The NOAEL dose of the Flemingia philippinensis Merr.et Rolfe was 60.6 g crude drug·kg-1(equivalent to 22.4～67.3 times that of adult dose).Conclusion Flemingia macrophylla(Willd.)Prain and Flemingia philippinensis Merr.et Rolfe are safe.
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