Qualitative analysis of chemical constituents of Guanxin Qiwei tablets based on HPLC-Q-Exactive-MS technique
Objective To rapidly and comprehensively determine the main components of Guanxin Qiwei tablets by high performance liquid chromatography-quadrupole/electrostatic field orbit-trap high resolution mass spectrometry(HPLC-Q-Exactive-MS).Methods The chromatography was performed on an Agilent ZORBAX SB-Aq column(4.6 mm×150 mm,5 μm)with mobile phase consisting of methanol(A)-0.5％formic acid water(B)at 1.0 mL·min-1.The column temperature was 35 ℃ and the sample size was 10 μL.Positive and negative ion scanning modes were used for the mass spectrometry.Scanning range of m/z was 110 to 1200.Based on the precise mass charge ratio and secondary mass spectrometry of the compounds,the analysis and identification of the components were conducted with the self-built database.Results Totally 67 compounds,including 30 flavonoids,18 organic acids,8 terpenoids,5 lignans,4 coumarins and 2 other compounds were identified in Guanxin Qiwei tablets.Conclusion The HPLC-Q-Exactive-MS method can rapidly and comprehensively analyze the chemical components in Guanxin Qiwei tablets,which provides reference for the quality control and research on pharmacodynamic substances.
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