舒肝宁注射液的1H-NMR指纹图谱分析
1H-NMR fingerprint of Shuganning injection
何峰 1朱彤彤 2韩春月 2张昀 3杨翠萍 3韩立峰 2付志飞2
作者信息
- 1. 贵州医科大学药学院,贵阳 550025
- 2. 天津中医药大学,天津市中药化学与分析重点实验室,天津 301617
- 3. 贵州瑞和制药有限公司,贵阳 550002
- 折叠
摘要
目的 对舒肝宁注射液的1H-NMR指纹图谱进行分析,为其质量控制标准技术升级提供参考依据.方法 使用 Noesygppr1d 脉冲序列[recycle delay(RD)-90°-t1-90°-tm-90°-ACQ]采集1H-NMR谱图,质子共振频率为600.13 MHz,实验温度为298 K,通过方法学考察验证分析方法可靠性,采用相关系数法和夹角余弦法评价不同批次舒肝宁注射液样品的相似度.结果 对舒肝宁注射液的1H-NMR指纹图谱进行分析,共鉴定出88种化学成分,且不同批次的舒肝宁注射液具有较高的相似度.结论 建立了一种基于1H-NMR的舒肝宁注射液质量控制方法,可为舒肝宁注射液技术升级提供参考依据.
Abstract
Objective To establish1H-NMR fingerprint of Shuganning injection and to provide reference for its quality control.Methods The 1H-NMR spectrum of the sample was collected by Noesygppr1d pulse sequence[recycle delay(RD)-90°-t1-90°-tm-90°-ACQ].The proton resonance frequency was 600.13 MHz and the measurement temperature was 298 K.The methodology was verified.Correlation method and Cosin method were used to evaluate the similarity.Results 1H-NMR fingerprint of Shuganning injection was established and 88 compounds were identified.Different batches of Shuganning injection were highly similar.Conclusion The 1H-NMR fingerprint of Shuganning is established,which provide some reference for the evaluation of the quality of Shuganning injections.
关键词
舒肝宁注射液/1H-NMR/指纹图谱/相似度Key words
Shuganning injection/1H-NMR/fingerprint/similarity引用本文复制引用
基金项目
贵州省科技计划(黔科合成果[2022]一般013)
贵州省科技计划(黔科合支撑[2023]一般158)
出版年
2024
NETL
NSTL
万方数据