HPLC specific chromatogram and quantitation of 4 components in Euryales semen formula granules
Objective To establish the HPLC specific chromatogram of Euryales semen formula granules and to determine the content of adenosine,gallic acid,guanosine and ellagic acid.Methods HPLC analysis of Euryales semen formula granules was conducted on Shim-pack GIST C18-AQ(4.6 mm×150 mm,3.0 μm)column with the mobile phase of acetonitrile-0.5％formic acid system in gradient elution mode at a flow of 1.0 mL·min-1.The ultraviolet detection wavelength was 258 nm and the column temperature was 20 ℃.The similarity was evaluated by the traditional Chinese medicine chromatography fingerprint map similarity system.Results Totally 9 Nine common peaks were identified in the characteristic chromatogram and 7 peaks were comfirmed.Peak 2 was adenosine,peak 3 was gallic acid,peak 4 was guanosine,peak 6 was brevifolin carboxylic acid,peak 7 was corilagin,peak 8 was urolithin M5,and peak 9 was ellagic acid.The similarity of multiple batches of samples was over 0.96.The content of adenosine,gallic acid,guanosine,and ellagic acid ranged 0.169～0.344 mg·g-1,0.469～1.956 mg·g-1,0.162～0.439 mg·g-1,and 0.101～0.617 mg·g-1.Conclusion The characteristic HPLC chromatogram of Euryales semen formula granules is simple repeatable,which can comprehensively and truly reflect the quality difference in Euryales semen formula granules.
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