Preparation optimisation and anti-neuroinflammation activity of liposomes chrysin
Objective To optimize the preparation of chrysin liposomes by response surface methodology and determine their in vitro anti-neuroinflammation activity.Methods The chrysin liposome was prepared by ethanol injection.According to the results of single factor experiment,the optimal prescription was screened and characterized by Box-Behnken response surface.Cytotoxicity experiments determined the safe drug concentration range of chrysin and chrysin liposome,and lipopolysaccharide(LPS)was used to analyze the anti-neuroinflammation activity in BV2 cells.Results Parameters for the optimal preparation of chrysin liposomes were as follows:lipid-cholestero 7.86∶1,stirring at 615 r·min-1,and temperature at 60 ℃.The morphology of chrysin liposome prepared under this condition was elliptical,with encapsulation rate(85.01±0.33)％,the average particle size(141.43±0.5)nm,and PDI(0.240±0.021).In vitro drug release showed obvious characteristics of sustained release.In vitro cell experiments indicated:the experimental concentration range of 0～100 μmol·L-blank liposomes without cells cytotoxicity on BV2 microglia.Anti-inflammatory performance indicated that as compared to free chrysin,chrysin liposomes had stronger anti-inflammatory effecting,and were no cytotoxicity within the range of use(＜25 μmol·L-1).Conclusion This study successfully prepares and optimizes chrysin liposomes,improves the solubility and release performance of chrysin in vitro,and showes good anti-inflammatory activity in vitro,providing a theoretical basis for the development of new formulations of chrysin.
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