Comparison sedative and hypnotic pharmacodynamics of hybrid Gastrodia elata and its parent alcohol extracts based on hypothalamic-pituitary-adrenal axis
Objective To compare the sedative and hypnotic effects of hybrid Gastrodia elata Blume and its parent alcohol extract based on hypothalamic-pituitary-adrenal(HPA)axis and monoamine neurotransmitter levels.Methods The insomnia model was established by intraperitoneal injection of p-chlorophenylalanine(PCPA)in mice for four consecutive days,and the drug was administered at the same time.The positive drug estazolam,hybrid Gastrodia elata and its parent alcohol extract(Gastrodia elata BL.f.glauca and Gastrodia elata BL.f.elata),and gastrodin were administered.Seven days later,the sleep incidence,sleep latency,and sleep time of mice induced by intraperitoneal injection of sodium pentobarbital were detected.Hematoxylin eosin staining was used to observe the lesions of brain related tissues in mice.ELISA method was used to detect the levels of monoamine neurotransmitters serotonin,5-hydroxytryptamine(5-HT),norepinephrine(NE)and dopamine(DA)in the body.HPA axis hormones in the body and serum y-aminobutyric acid(GABA)levels were also measured.Results The mice model of PCPA induced insomnia mice was successfully established.Gastrodia alcohol extract of each group improved the behavioral abnormalities in insomnia mice,alleviated the brain tissue lesions of insomnia mice,and promoted the callback of 5-HT and DA expression in the body;the increase of NE level in the serum,corticotropin releasing hormone(CRH),adrenocorticotropic hormone(ACTH)and corticosterone(CORT)levels in the body were inhibited,and GABA level increased in the body.The regulatory effect of hybrid Gastrodia elata on monoamine neurotransmitters was better than that of Gastrodia elata BL.f.elata.The effect of inhibiting HPA axis hyperactivity and increasing GABA level in the body was better than that of Gastrodia elata BL.f.glauca.Conclusion Both hybrid Gastrodia elata and its parent ethanol extract can alleviate insomnia by inhibiting HPA axis hyperactivity and regulating monoamine neurotransmitter disorders in the body.The comprehensive efficacy of hybrid Gastrodia elata is similar to that of its parents,and reference data can be provided for subsequent pharmacodynamic studies.
hybrid Gastrodia elatasedative and hypnoticdifference in efficacyHPA axis5-HT
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