Active ingredients and mechanism of Rhubarb-Peach kernel pair in removing blood stasis and purging heat based GC-MS and network pharmacology
Objective To analyze the chemical compositions of the decoction of Rhubarb-Peach kernel pair,and to determine the pharmacodynamic substances and mechanism of action in removing blood stasis and purging heat.Methods UPLC-TOF-MS and GC-MS were used to analyze the composition of Rhubarb-Peach kernel pair decoction.Network pharmacology was used to establish the"disease-component-target"network of Rhubarb-Peach kernel pair and screen the target signaling pathways.The rat model of stasis-heat was replicated by tail vein injection of endotoxin lipopolysaccharide twice at an interval of 24 hours.The efficacy of Rhubarb-Peach kernel pair was evaluated with changes in body temperature,blood rheology,four coagulation items(TT,PT,APTT,and FIB),inflammatory factor levels,and oxidative stress levels in rats.The predicted core targets were verified by molecular docking and real-time fluorescence quantitative polymerase chain reaction.Results Totally 64 compounds of Rhubarb-Peach kernel pair decoction were identified.Network pharmacological indicated that the core components of Rhubarb-Peach kernel pair,such as aloe-emodin,eriodictyol,epigallocatechin gallate,and procyanidin modulated the pathways of VEGF,MAPK,IL-17,and TNF to remove blood stasis and purge heat through the core targets,such as AKT1,PTGS2,SRC,IL1B and TNF.Docking between the active ingredients and core targets molecules were favorable.The animal experiments showed that after the Rhubarb-Peach kernel pair treatment,the oxidative stress(NO,SOD,and MDA),four coagulation items,blood rheology,body temperature and other indicators of the blood of stasis-heat model rats all returned to normal levels.Rhubarb-Peach kernel pair reduced the contents of inflammatory factors IL-1β,IL-17,and TNF-α,as well as the expression of core targets,namely AKT1,SRC,MYC,EGFR,PTGS2,and TP53 mRNA.Conclusion Compounds such as eriodictyol,aloe-emodin,procyanidin,catechin gallte,and epigallocatechin gallate are the material basis for Rhubarb-Peach kernel pair to remove blood stasis and purge heat.Through multi-target and multi-pathway regulation of the inflammation,oxidative stress,angiogenesis and vascular endothelium,Rhubarb-Peach kernel pair helps remove the blood stasis and purge heat.
Rhubarb-Peach kernel pairremove blood stasis and purge heatUPLC-TOF-MSGC-MSnetwork pharmacologypharmacodynamic experiment
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