Identification and cloning of a 2,3-oxidosqualene cyclase gene LaOSC1 from Lantana camara
Objective To identify and clone candidate 2,3-oxidosqualene cyclase gene,a rate-limiting enzyme involved in the biosynthetic pathway of active triterpenoid components,in the medicinal plant Lantana camara.Methods Candidate squalene 2,3-oxidosqualene cyclase gene was identified from transcriptome data of Lantana camara based on sequence similarity comparison.Its sequence characteristics,biological characteristics of its coding protein,and evolutionary relationship were analyzed by bioinformatics softwares.Specific primers were designed to amplify the squalene 2,3-oxidosqualene cyclase gene from cDNA of Lantana camara and construct the clone vector.Results A 2,3-oxidosqualene cyclase gene LaOSC1 with the conserved domain of OSC gene was successfully identified,whose coding protein might catalyze the formation of beta-amyrin from 2,3-oxidosqualene cyclase.The phylogenetic tree suggested that it might encode β-amyrin synthase protein,and the cloning vector of LaOSC1 was successfully constructed.Conclusion LaOSC1,a 2,3-oxidosqualene cyclase gene from Lantana camara,is successfully identified and cloned,which lays a foundation for the subsequent study of the biosynthetic pathway of active components in Lantana camara.
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