Inhibition effect of Compound Gegen decoction on the development of colon cancer in vivo by regulating IL-6/STAT3 and ferroptosis pathway
Objective To determine the molecular mechanism of Compound Gegen decoction(CGD)inhibiting colon cancer in vivo.Methods The HCT116 cell-derived tumor bearing mouse model was established and daily given CGD or equal volume saline intragastrically at the dose of 1.0 g·kg-1 for 23 days,and the weight and tumor size were measured once every three days.The ferroptosis pathway,IL-6 pathway and cell migration-related proteins were detected by Western blot.Results Compared with the control group,CGD greatly inhibited the growth of HCT116 cell-derived tumor in vivo.Ferroptosis-related proteins ACSL4 and PTGS2 were much up-regulated,while SLC7A11 and GPX4 were obviously down-regulated,and IL-6,p-STAT3/STAT3 ratio and its downstream proteins MMP2,TWIST and MMP7 were all greatly down-regulated.Conclusion CGD can inhibit the growth of colon cancer in vivo,whose mechanism may be through inhibiting IL-6/STAT3 and inducing ferroptosis pathway.
Compound Gegen decoctioncolon cancer in vivoIL-6/STAT3 pathwayferroptosis
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