复方葛根汤调控IL-6/STAT3和铁死亡通路抑制在体结肠癌发展
Inhibition effect of Compound Gegen decoction on the development of colon cancer in vivo by regulating IL-6/STAT3 and ferroptosis pathway
钱玲 1赵斌 1饶江泉 1徐恩惠 1陈慧 2杨潮 2罗小泉 1陈来3
作者信息
- 1. 江西中医药大学,南昌 330004
- 2. 江西中医药大学,南昌 330004;江西省癌症技术转化工程研究中心,南昌 330004
- 3. 江西中医药大学,南昌 330004;江西省中药药理重点实验室,南昌 330004;江西省癌症技术转化工程研究中心,南昌 330004
- 折叠
摘要
目的 探究复方葛根汤在体抗结肠癌的分子机制.方法 建立HCT116细胞荷瘤小鼠模型,每日以1.0g·kg-1的剂量灌胃给予复方葛根汤或等体积生理盐水,每3日测量一次体重和瘤体大小,连续给药23 d;Western blot检测铁死亡通路、IL-6通路和细胞迁移相关蛋白.结果 与对照组相比,复方葛根汤显著抑制HCT116细胞衍生瘤体增长,显著上调铁死亡相关蛋白 ACSL4、PTGS2,而明显下调 SLC7A11、GPX4,还显著下调 IL-6、p-STAT3/STAT3 比及其下游蛋白MMP2、TWIST和MMP7水平.结论 复方葛根汤能显著抑制体内结肠癌发展,其机制可能为通过抑制IL-6/STAT3和诱导铁死亡通路.
Abstract
Objective To determine the molecular mechanism of Compound Gegen decoction(CGD)inhibiting colon cancer in vivo.Methods The HCT116 cell-derived tumor bearing mouse model was established and daily given CGD or equal volume saline intragastrically at the dose of 1.0 g·kg-1 for 23 days,and the weight and tumor size were measured once every three days.The ferroptosis pathway,IL-6 pathway and cell migration-related proteins were detected by Western blot.Results Compared with the control group,CGD greatly inhibited the growth of HCT116 cell-derived tumor in vivo.Ferroptosis-related proteins ACSL4 and PTGS2 were much up-regulated,while SLC7A11 and GPX4 were obviously down-regulated,and IL-6,p-STAT3/STAT3 ratio and its downstream proteins MMP2,TWIST and MMP7 were all greatly down-regulated.Conclusion CGD can inhibit the growth of colon cancer in vivo,whose mechanism may be through inhibiting IL-6/STAT3 and inducing ferroptosis pathway.
关键词
复方葛根汤/在体结肠癌/IL-6/STAT3通路/铁死亡Key words
Compound Gegen decoction/colon cancer in vivo/IL-6/STAT3 pathway/ferroptosis引用本文复制引用
基金项目
江西省卫生与健康委员会科技项目(202211419)
江西省科技计划(20212BCD46005)
江西省中医药局科技项目(2022B1051)
出版年
2024
NETL
NSTL
万方数据