Extraction of Jiangzhi Hugan prescription based on concept of quality by design
Objective To optimize the extraction of Jiangzhi Hugan prescription(JZHG)based on the concept of quality by design.Methods The number of extractions,extraction time and the times of water addition were used as the critical process parameters(CPPs).The orthogonal design was adopted to screen the critical quality attributes(CQAs)corresponding to CPPs from four potential CQAs,namely the content of astragaloside Ⅳ,betaine,polysaccharide and the extraction amount of solid matter.The CQAs were determined by Box-Behnken design.The mathematical model was established to determine the interaction between CPPs and CQAs.The design space of the JZHG extraction was established and verified.Results The content of betaine and the extraction amount of solid matter were determined as CQAs in the extraction by the variance analysis of Box-Behnken experiment.Through the optimization of the design space,the optimum extraction process was obtained as follows:adding 10 times of water,3 extractions,and each extraction 1.5 h.Conclusion The optimized extraction is stable and reliable,which provides reference for the process development and quality control of JZHG.
quality by designJiangzhi Hugan prescriptionextraction processBox-Behnken designdesign space
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