Quality evaluation of seeds of Dimocarpus longan Lour.based on HPLC fingerprint and multi-component content determination combined with chemical pattern recognition
Objective To establish the HPLC fingerprint of seeds of Dimocarpus longan Lour.and to determine differential components.Methods The quality of seeds of Dimocarpus longan was analyzed by establishing the fingerprints of 13 batches of samples combined with similarity evaluation of the fingerprints and chemical pattern recognition.The content of gallic acid,corilagin,and ellagic acid was determined.Results The similarity of 13 batches of seeds of Dimocarpus longan ranged from 0.919 to 0.999,with a total of 11 peaks confirmed and 3 components identified.HCA clustered 13 batches of seeds of Dimocarpus longan into 3 categories,PCA extracted 3 principal components,and OPLS-DA screened 5 differential components.Quantitative analysis showed that the content ranges of gallic acid,corilagin,and ellagic acid were 0.65～1.8 mg·g-1,3.02～5.46 mg·g-1,and 3.18～4.49 mg·g-1,respectively.Conclusion The HPLC fingerprint and content determination method are stable and repeatable,which can provide reference for the quality evaluation of seeds of Dimocarpus longan.
seeds of Dimocarpus longan Lour.fingerprintchemical pattern recognitioncontent determination
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