Determination of α-lipoic acid in α-lipoic acid with green tea tablets by high-performance liquid chromatography
Objective To establish a method for the determination of α-lipoic acid in α-lipoic acid with green tea tablets.Methods HPLC was carried out,and the experiment was conducted with an Alltima C18 column(250 mm × 4.6 mm,5 μm).The mobile phase was acetonitrile-0.1％phosphoric acid solution(55∶45)at the flow rate of 1.0 mL·min-1.The detection wavelength was 215 nm and the column temperature was 30 ℃.Results α-lipoic acid showed a good linearity with the peak area at 102.6～307.7 μg·mL-1,and the correlation coefficient(r)was 1.000.The RSD of precision,repeatability and stability were less than 2.0％.The average recovery of 3 concentrations was 98.44％～100.2％,and the relative standard deviation was less than 1.0％.Conclusion The method is fast,simple and accurate,and can be used to determine α-lipoic acid in α-lipoic acid with green tea tablets.
α-lipoic acidα-lipoic acid with green tea tabletHPLCdetermination
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