高效液相色谱法测定α-硫辛酸绿茶片中α-硫辛酸的含量
Determination of α-lipoic acid in α-lipoic acid with green tea tablets by high-performance liquid chromatography
徐硕 1王月 1邝咏梅 1徐文峰 1金鹏飞1
作者信息
- 1. 北京医院药学部,国家老年医学中心,中国医学科学院老年医学研究院,北京市药物临床风险与个体化应用评价重点实验室(北京医院),北京 100730
- 折叠
摘要
目的 建立α-硫辛酸绿茶片中α-硫辛酸的含量测定方法.方法 利用高效液相色谱(HPLC)技术,采用Alltima C18柱(250 mm×4.6 mm,5 μm),流动相为乙腈-0.1％磷酸溶液(55∶45),流速为1.0 mL·min-1,检测波长为215 nm,柱温为30 ℃.结果 α-硫辛酸在102.6～307.7 μg·mL-1与峰面积呈良好的线性关系,相关系数(r)为1.000;精密度、重复性和稳定性考察RSD均小于2.0％;3个浓度的加样回收率为98.44％～100.2％,RSD值均小于1.0％.结论 该方法准确、简单、快速,能够用于α-硫辛酸绿茶片中α-硫辛酸的含量测定.
Abstract
Objective To establish a method for the determination of α-lipoic acid in α-lipoic acid with green tea tablets.Methods HPLC was carried out,and the experiment was conducted with an Alltima C18 column(250 mm × 4.6 mm,5 μm).The mobile phase was acetonitrile-0.1％phosphoric acid solution(55∶45)at the flow rate of 1.0 mL·min-1.The detection wavelength was 215 nm and the column temperature was 30 ℃.Results α-lipoic acid showed a good linearity with the peak area at 102.6～307.7 μg·mL-1,and the correlation coefficient(r)was 1.000.The RSD of precision,repeatability and stability were less than 2.0％.The average recovery of 3 concentrations was 98.44％～100.2％,and the relative standard deviation was less than 1.0％.Conclusion The method is fast,simple and accurate,and can be used to determine α-lipoic acid in α-lipoic acid with green tea tablets.
关键词
α-硫辛酸/α-硫辛酸绿茶片/高效液相色谱/含量测定Key words
α-lipoic acid/α-lipoic acid with green tea tablet/HPLC/determination引用本文复制引用
出版年
2024
NETL
NSTL
万方数据