Determination of aloe-emodin related substances by HPLC
Objective To determine the related substances in aloe-emodin by HPLC.Methods A Welch Xtimate C18 column(150 mm × 4.6 mm,5 μm)was used with a mobile phase consisted of pH 2.0 phosphate buffer-methanol-acetonitrile in the gradient elution.The flow rate was maintained at 1.2 mL·min-1,with an injection volume of 20 μL,a column temperature of 30 ℃,and detection wavelength at 254 nm.Results Aloe-emodin and its related substances were well separated.Experiments revealed excellent precision,with a maximum relative standard deviation(RSD)below 13％when the impurity content was below 0.1％.When the impurity content≥0.1％,the maximum RSD among the 12 parts tested was below 4.0％.The predominant single impurity as rhubarb aldehyde was identified in 6 batches of aloe-emodin samples from the same manufacturer,with a content of 1.42％.The total impurity content in these samples was less than 5.0％,with variations in the impurity profiles among different batches.Conclusion This method is sensitive,specific,and accurate,which is suitable for the detection of related substances in aloe-emodin and control its quality.
aloe-emodinHPLCmuti-component content determinationquality control
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