Preparation of celastrol-loaded hollow mesoporous polydopamine nanocomposite and its in vitro antitumor activity
Objective To prepare hollow mesoporous polydopamine nanocomposites loaded with celastrol(H-PDA/Cel@HA)and investigate in vitro antitumor activity.Methods The were synthesized via a wet chemical etching method.The nanocomposites were characterized using techniques such as particle size analysis,Zeta potential measurement,transmission electron microscopy,and ultraviolet absorption spectroscopy.The antitumor activity of H-PDA/Cel@HA against breast cancer 4T1 cells was assessed through cytotoxicity assays,evaluation of cellular reactive oxygen species(ROS)levels,live/dead cell staining experiments,and apoptosis analysis.Results The H-PDA/Cel@HA exhibited a hollow cubic morphology,with an average particle size of(183.4±12.3)nm.The Zeta potential values of H-PDA/Cel@HA was(-30.4±1.1)mV.The drug loading rate was(13.25±1.16)％,with an encapsulation rate of(66.27±5.78)％.H-PDA/Cel@HA demonstrated significant inhibition of breast cancer 4T1 cell growth and exhibits better biocompatibility with normal cells(L929)compared to free Cel.Moreover,H-PDA/Cel@HA increased the ROS levels in 4T1 cells and induced apoptosis.Conclusion H-PDA/Cel@HA exhibit promising biocompatibility,exhibit promising biocompatibility,tumor-targeted delivery ability,and can effectively inhibit the growth of tumor cells,and can effectively inhibit the growth of tumor cells.
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