Preparation and synergistic antimicrobial effect of submicroemulsion of Cinnamon oil-Artemisia argyi oil complexes
Objective To determine the single and combined antibacterial activity of Cinnamon oil,Artemisia argyi oil and Cinnamon-Artemisia argyi oil,optimize the submicroemulsion formula and process of Cinnamon oil-Artemisia argyi oil,and evaluate its quality.Methods The minimum inhibitory concentration(MIC)and minimal bactericidal concentration(MBC)of Cinnamon oil and Artemisia argyi oil against S.aureus,B.subtilis,and E.coli were determined by microdilution method.The fractional inhibitory concentration(FIC)of the two volatile oils in combination was determined by checkerboard dilution method.The diameter of the circle of inhibition under different mixing ratios of the oils was determined by filter paper slice method,and the centrifugal stability constants,particle sizes and polydispersity index(PDI)were used as the evaluation indexes for the optimal formula of the submicroemulsion.Results The MIC of Cinnamon oil was 0.5 μL·mL-1 for S.aureus,1 μL·mL-1 for B.subtilis and E.coli,and 16 μL·mL-1 for Artemisia argyi oil for the three test organisms.Cinnamon oil and Artemisia argyi oils interacted with S.aureus and had synergistic effect on E.coli and B.subtilis.The optimal formula of compound submicroemulsion was Cinnamon oil-Artemisia argyi oil-soybean phospholipid-Poroxam 188(P188)-water at 2.5∶3∶0.2∶0.8∶93.5.The resulting submicroemulsion was a milky white liquid with an average particle size of(130.7±1.2)nm and the PDI of 0.145±0.02.Conclusion The Cinnamon oil-Artemisia argyi oil complex submicroemulsion can inhibit S.aureus,B.subtilis and E.coli,whose effect is stronger than that of volatile oil.
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