Chemical components analysis of compound Muji mixture based on UPLC-Q-TOF-MS/MS technology
Objective To determine the components in compound Muji mixture by UPLC-Q-TOF-MS/MS,and to provide a basis for the quality control of compound Muji mixture.Methods Compound Muji mixture was analyzed with Agilent Poroshell SB-C18 chromatography column(100 mm × 4.6 mm,2.7μm)for gradient elution with the mobile phase of 0.1％formic acid water solution-acetonitrile solution as positive ion modes,water-acetonitrile solution as negative ion modes at a flow rate 0.3 mL·min-1.The column temperature was 30 ℃ and the injection volume was 2 μL.Electriospray ion(ESI)source was used in the analysis under the positive and negative ion modes.The detected chemical components were identified via comparison with reference materials,precise molecular weight,and fragmentation pathways by secondary mass spectrometry.Based on literature reports,the structural formula of the chemical components was determined.Results Totally 34 chemical components were identified in compound Muji mixture,including 11 alkaloids,11 organic acids,5 flavonoids,1 naphthoquinone,1 phenolic,1 nucleoside,and 4 other compounds.Organic acids and alkaloids were the main components.Conclusion The compositions in compound Muji mixture are clarified,which provides an experimental basis for its pharmacodynamics,quality control and mechanism of action.
NETL
NSTL
万方数据
