Anti-liver fibrosis components of Ganshuang granule based on UPLC-Q-TOF-MS/MS and liver fibrosis cell model
Objective To screen the liver fibrosis pharmacodynamic constituents of Ganshuang granules by multi-technique approach.Methods UPLC-Q-TOF-MS/MS technology and UNIFI software were used to analyze the mass spectrometry fragmentation information of the chemicals in the 70％alcoholic extract of Ganshuang granules and in rat serum after gavage according to mass spectrometry relative molecular mass,cleavage pattern,and literature reports,to clarify the pharmacological substance basis for the drug effect.Liver fibrosis cell model was constructed using transforming growth factorβ1(TGF-β1)(10 ng·mL-1)-induced hepatic stellate cells(HSC-T6),and the cell proliferation was detected by cell proliferation assay(CCK-8)method in each group.Results Totally 84 chemical components in the extract of Ganshuang granules,and 21 prototypical components were identified in the serum of rats after the drug administration.Among them,codonopsine,saikosaponin A,saikosaponin D,luteolin,kaempferol,and baicalin all inhibited the growth of HSC,and were the main components of Ganshuang granules for their anti-liver fibrosis pharmacological efficacy.Conclusion The pharmacological basis of anti-liver fibrosis of Ganshuang granules is made clear,which provides a basis for the research on the mechanism of hepatoprotection of Ganshuang granules and the quality control of the product.
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