Puerarin decreases IL6 expression and inhibits cell proliferation in bladder urothelial carcinoma T24 cells
Objective To determine the mechanism and potential targets of puerarin,when inhibiting the proliferation of bladder urothelial carcinoma T24 cells.Methods The inhibitory effect of puerarin on the proliferation of T24 cells was demonstrated by CCK8 assay.The differentially expressed gene list(DEGL)was obtained by gene expression microarray.Volcano maps and heat maps were used for the quality control of chip data.KEGG,GO pathway enrichment analysis,protein-protein interaction(PPI)and upstream transcription factor prediction were performed to obtain key targets and pathways,and then further validated them.Results Puerarin inhibited the proliferation of T24 cells,whose inhibitory effect was proportional to its concentration,and the half inhibitory concentration was 218 μmol·L-1.KEGG pathway enrichment analysis showed that DEGL was mainly enriched in cell growth and proliferation-related pathways,and GO showed that DEGL was enriched in protein folding,DNA replication and tumor cell necrosis-related pathways.PPI yielded the key target IL6.The expression of IL6 in stage Ⅲ and Ⅳ was obviously higher than that in stageⅡ,and PCR experiments showed that puerarin reduced the expression of IL6 in T24 cells.GSEA analysis showed that IL6 was associated with epithelial cell proliferation.CENPA was predicted by upstream transcription factors,and the molecular docking showed good docking between puerarin and CENPA.Conclusion Puerarin can inhibit the proliferation of T24 cells,whose mechanism may be via the binding of puerarin and CENPA to reduce the expression of IL6 and affect protein folding,DNA replication,tumor cell necrosis and proliferation-related pathways,and ultimately inhibit T24 cell proliferation.
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