Nano-sized LDH as carriers for adsorption and loading of gambogic acid:experimental and molecular docking simulation
Objective To obtain GA-LDH nanohybrid with the gambogic acid(GA)adsorped to the surface of layered double hydroxides(LDH).Methods A HPLC method for the determination of GA was established and validated.GA-LDH nanohybrid was prepared by co-precipitation,ion exchange,exfoliation recombination and miceller drug loading.The drug loading rate and encapsulation effiency were used as the indexes,the drug-loading process of GA-LDH nanohybrid was determined by single factors,such as drug-loading method,drug/LDH ratio and particle size of LDH.The samples were structurally characterized by IR and XRD.The buffering and sustained release properties of GA-LDH were investigated.The molecular docking method was used to calculate the magnitude of binding energy between GA and LDHs.Results The optimum preparation process of GA-LDH nanohybrid was as follows:ion exchange method,drug loading ratio of 3∶5 and hydrothermal LDH as the carrier.The drug loading rate and encapsulation effiency of GA-LDH were 21.37％and 46.29％under optimized conditions.Acid base titration showed that GA-LDH had similar buffer performance to LDH.In vitro release experiments showed that GA-LDH had a certain sustained-release effect,whose mechanism was a passive diffusion process.The molecular docking method theoretically confirmed the experimental results.Conclusion The drug-loaded complex has good drug-loading and buffering properties,which is expected to be a new type of efficient carrier for antitumour components of traditional Chinese medicine.
NETL
NSTL
万方数据
