Active ingredients in Radix Rehmanniae Praeparata to promote the proliferation of neural stem cells
Objective To determine the active components in Radix Rehmanniae Praeparata to promote the proliferation of neural stem cells(NSCs)in vitro.Methods The system solvent extraction method and column chromatography were used to separate the various parts of Radix Rehmanniae Praeparata.CCK-8 assay was used to determine the effect of the compounds obtained from different chemical separation sites and active sites of Radix Rehmanniae Praeparata on the proliferation of NSCs.Results After water extraction and alcohol precipitation of Radix Rehmanniae Praeparata,the water position was separated by macroporous resin,silica gel,and polyamide to obtain the FrC5 part.The FrC5 part significantly promotied the proliferation of NSCs,the difference was significant as compared with the control group(P＜0.05).The FrC5 part was divided into FrC5-1 and FrC5-2 according to solubility,the FrC5-1 site showed stronger effect compared with that of other groups with significant difference(P＜0.05).Compared with the control group,the proliferation rate of NSCs was 75.85％.Two compounds were further separated by MS and NMR spectrum data and references,which were acteoside and isoacteoside.The proliferation rate of acteoside on NSCs was 65.04％,while that of isoacteoside on NSCs was 35.86％.Conclusion Acteoside and isoacteoside may be the main active components in Radix Rehmanniae Praeparata to promote the proliferation of NSCs.
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