Preparation and properties of astragaloside loaded by y-cyclodextrin metal-organic framework
Objective To prepare γ-cyclodextrin metal-organic framework(CD-MOF)containing astragaloside(AST)with CD-MOF as the carrier and to determine its effect on AST solubility,dissolution rate,stability and apoptosis of A549 cells.Methods AST was loaded into CD-MOF by solvent incubation method.The morphology and adsorption properties of AST@CD-MOF were characterized by environmental scanning electron microscopy and nitrogen adsorption.Meanwhile,the solubility,dissolution rate,stability and induction of A549 cell apoptosis of AST@CD-MOF were measured before comparion with those of AST@y-CD and AST powder.Results The morphology of AST@CD-MOF was homogeneous cubic crystal with a particle size of about 140 nm.The drug loading of optimized AST@CD-MOF was(32.29±2.57)％,with good stability.The dissolution rate and cumulative dissolution rate of AST in AST@CD-MOF was obviously improved,and the cumulative dissolution percentage reached over 80％within 6 h.The total apoptosis rate induced by AST@CD-MOF was 68.18％,much higher than that induced by free AST and AST@y-CD.Conclusion CD-MOF can greatly increase the water solubility and dissolution rate of AST,and enhance the ability of AST to induce the apoptosis of A549 cells.
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