Mechanism of Sophora flavescens in the topical treatment of atopic dermatitis by UPLC-Q-TOF-MS combined with network pharmacology
Objective To determine the skin retention components and blood components in the alcohol extract of Sophora flavescens after transdermal administration,and its potential mechanism for atopic dermatitis.Methods UPLC-Q-TOF-MS was used to qualitatively analyze the skin retention components and blood components in mice.The targets of chemical components and disease were obtained through Swiss Target Prediction,GeneCards and OMIM databases.PPI network was established through STRING database and Cytoscape software.Gene Ontology(GO)function analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis were conducted by DAVID database.Results Totally 18 prototype skin retention components and 4 prototype blood components were identified.The network pharmacology showed that the key targets included signal transducer and activator of transcription(STAT)3,protein kinase B(AKT)1,etc.KEGG enrichment analysis mainly involved phosphoinositide 3-kinases(PI3K)-AKT and other signal pathways.Conclusion The pharmacodynamic basis of Sophora flavescens is determined by UPLC-QTOF-MS.Its potential mechanism for atopic dermatitis is made clear by network pharmacology,providing reference for the development and utilization of Sophora flavescens.
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