HPLC fingerprint and content determination of 5 components in Tibetan medicine Wuwei Qingre decoction powder
Objective To establish the HPLC fingerprint of the Tibetan medicine Wuwei Qingre decoction powder and determine the content of gallic acid,methyl gallate,chebulate tannic acid,chebulate acid and ellagic acid.Methods An Ultimate XB-C18 liquid chromatographic column(4.6 mm × 250 mm,5 μm)was used,with 0.1％phosphoric acid aqueous solution(A)-methanol(B)as the mobile phase,gradient elution,flow rate of 1 mL·min-1,detection wavelength of 270 nm,column temperature of 30 ℃,and injection volume of 10 μL.Results Totally 18 common peaks in the fingerprint of Wuwei Qingre decoction powder were confirmed,and the similarity of fingerprint was higher than 0.99,The linear ranges of gallic acid,methyl gallate,chebulate tannic acid,chebulate acid,and ellagic acid were 2.76～55.17 μg·mL-1(r=0.9998),1.56～31.13 μg·mL-1(r=0.9997),5.51～110.17 μg·mL-1(r=0.9999),7.27～145.31 μg·mL-1(r=0.9998),and 0.90～18.08μg·mL-1(r=0.9997),respectively.The RSDs of precision,repeatability and stability tests were all less than 2.0％(n=6).The average recoveries were 98.27％(RSD=0.72％),98.31％(RSD=0.94％),96.48％(RSD=0.73％),96.86％(RSD=0.80％)and 96.07％(RSD=0.81％)respectively.The content of the 5 components in 8 batches sample was 7.85～8.25,4.02～4.23,16.85～17.77,23.55～24.51,and 3.09～3.18 mg·g-1,respectively.Conclusion The fingerprint and content determination method is simple,accurate,stable and rapid,which can provide reference for the quality control of Wuwei Qingre decoction powder.
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