Quantitative detcetion of bacterial endotoxin content in fetal bovine serum by micro-kinetic chromogenic method
Objective To determine the feasibility of quantitatively detecting the bacterial endotoxin in fetal bovine serum by micro-kinetic chromogenic method and compare the results with those of gel-clot method.Methods The micro-kinetic chromogenic method of limulus reagent was used to establish a bacterial endoxtoxin standard curve.The dilution factor was determined through interference pre-experiment,and the recovery rate of the endotoxin added to the test solution was determined.The interference test was performed to quantitatively detected the bacterial endotoxin content in the test product,and the results were compared with those of the gel-clot method.Results The linear range of the standard curve was 0.02 to 20 EU·mL-1,the regression equation was lgT=2.9334-0.2883 lgC,and the correlation coefficient |r|=0.9992.The samples had no interference when being diluted 200 times,the recovery rate of bacterial endotoxin ranged from 50％to 200％.The result of endotoxin quantitative detection for 3 batches of samples was lower than the specified limit,and that of 1 batch was higher than the limit.Conclusion Micro-kinetic chromogenic method for quantitative detection of the bacterial endotoxin can be used for the quality control of fetal bovine serum.
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