HPLC-MS/MS method for the simultaneous determination of vancomycin and digoxin in children's plasma
Objective To establish an HPLC-MS/MS method for the simultaneous determination of vancomycin and digoxin in children's plasma,and to monitor children therapeutic drugs.Methods The plasma sample protein were precipitated by methanol with the norvancomycin and digoxin-d3 as the intemal standards.The analytes were gradient eluted on an EVO-C18 column by HPLC-MS/MS,with the mobile phase consisting of water(0.02％formic acid and 2 mmol·L-1 ammonium acetate)-methanol at the flow rate of 0.55 mL·min-1.Ions were monitored in the dynamic multiple reaction monitoring(dMRM)mode,with positive ion electrospray ionization(ESI).Results Calibration plots were established over the concentration range of 1～50 μg·mL-1 for vancomycin(R2＞0.991),while 0.25～8 ng·mL-1 for digoxin(R2＞0.993).The accuracy,precision,matrix effect and stability all met the requirements.This method was fully verified and used to determine the drug concentrations in 311 plasma samples.Conclusion The method is simple,rapid,sensitive and suitable to monitorthe vancomycin and digoxin in children's plasma.
vancomycindigoxinHPLC-MS/MStherapeutic drug monitoringchildren
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