Brain,intestinal and feces components of Jingning formula based on UHPLC-Q-Exactive Orbitrap MS technology
Objective To identify and analyze the components in the brain,intestine and feces of rats after the administration of Jingning formula by UHPLC-Q-Exactive Orbitrap MS technology.Methods UHPLC-Q-Exactive Orbitrap MS was used.The mobile phase was 0.1%formic acid-acetonitrile(A)and 0.1%formic acid-water(B)with gradient elution,the flow rate was 0.2 mL·min-1,and the injection volume was 5 μL.The mass spectrometry conditions were included both positive and negative ion scannings,and the ion source was heated with electrospray ionization.The chemical compositions were analyzed by comparison with reference materials,literature reports and databases.Results Totally 42 components were identified,of which totally 9,33,20,and 27 components were identified in the brain,ileum,colon and feces,respectively.Conclusion The compositions of Jingning formula into the brain,intestine and feces are elucidated,which provides a basis for clarifying the material basis and action mechanism of Jingning formula.
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