基于LC-MS/MS法的芍药苷在正常和肝内胆汁淤积大鼠体内的药代动力学比较研究
Pharmacokinetics of paeoniflorin in normal and intrahepatic cholestatic rats by LC-MS/MS
杨梦换 1蒋罡 1陈卫东 1张善堂 2王汝琳1
作者信息
- 1. 安徽中医药大学药学院,合肥 230012;省部共建安徽道地中药材品质提升协同创新中心,合肥 230012
- 2. 安徽中医药大学药学院,合肥 230012;安徽省立医院药剂科,合肥 230001
- 折叠
摘要
目的 比较正常和 17α-乙炔基雌二醇诱导的胆汁淤积性肝损伤大鼠体内芍药苷的药代动力学过程的差异.方法 采用LC-MS/MS法检测给药后不同时间点大鼠血浆样本中芍药苷的含量,DAS 2.0 软件测算两组的药代动力学参数,并进行统计学分析.结果 与正常大鼠相比,胆汁淤积性肝损伤发生后,芍药苷的t1/2Z、MRT0～∞、AUC和Vz/F明显增加,而CLz/F显著降低.结论 与正常大鼠相比,胆汁淤积模型大鼠体内芍药苷的浓度更高、消除速度更缓慢.
Abstract
Objective To compare the pharmacokinetic process of paeoniflorin in normal rats and rats with 17α-ethinylestradiol-induced cholestatic liver injury.Methods The content of paeoniflorin in the rat plasma samples at different time points after the administration was detected by LC-MS/MS.The pharmacokinetic parameters of the two groups were analyzed by DAS 2.0 software.Results The pharmacokinetic behaviors of rats changed after the development of cholestatic liver injury as compared to those of normal rats.Specifically,t1/2Z,MRT0～∞,AUC and Vz/F of paeoniflorin were obviously increased,while CLz/F was greatly decreased.Conclusion Compared with the normal rats,the concentration of paeoniflorin is higher and the elimination rate slower in the cholestatic model rats.
关键词
芍药苷/17α-乙炔基雌二醇/胆汁淤积性肝损伤/药代动力学Key words
paeoniflorin/17α-ethinylestradiol/cholestatic liver injury/pharmacokinetics引用本文复制引用
基金项目
国家自然科学基金青年科学基金(82104517)
出版年
2024
NETL
NSTL
万方数据