UPLC-Q-TOF-MS combined with network pharmacology for the mechanism of action of Sophora flavescens in the treatment of eczema
Objective To determine the potential mechanism of transdermal administration of Sophora flavescens on eczema at molecular level with UPLC-Q-TOF-MS and network pharmacology.Methods The chemical compositions were identified by UPLC-Q-TOF-MS technique and literature data.The target was predicted by Swiss Target Prediction database,the protein interaction(PPI)network map was established by Cytoscape 3.9.0,and the target was analyzed by GO annotation and KEGG pathway with Metascape database.Results After transdermal administration of the drug in mice,14 components of the skin retention prototype and 7 components of the blood prototype were inferred.The core targets of Sophora flavescens treatment for eczema,such as tumor necrosis factor,vascular endothelial growth factor A and epidermal growth factor receptor,were identified by network pharmacology.KEGG enrichment analysis showed close relation to serotonergic synaptic signaling pathway and arachidonic acid metabolism.Conclusion UPLC-Q-TOF-MS combined with network pharmacology is used to further elucidate the main targets and pathways of Sophora flavescens for the treatment of eczema.
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