Construction of salidroside liposomes and their protective effects on oxidative damage in ARPE-19 cells induced by high glucose
Objective To construct salidroside liposomes(SAL-LIPS),evaluate their protective effects against oxidative damage in human retinal pigment epithelial(ARPE-19)cells under high glucose conditions.Methods SAL-LIPS were prepared with ethanol injection method.The morphology was observed with transmission electron microscopy,and particle size,polydispersity index(PDI),as well as in vitro release and stability assessed.An oxidative stress cell model was established in ARPE-19 cells induced by high glucose.The effect of different concentrations of SAL-LIPS on the viability of damaged cells was examined,and the expression levels of reactive oxygen species(ROS),malondialdehyde(MDA),vascular endothelial growth factor(VEGF),and hypoxia-inducible factor(HIF-1α)in different groups were measured.Results The prepared SAL-LIPS were spherical with an average particle size of(112.1±2)nm and an average PDI of 0.204±0.02,demonstrating a sustained-release effect and better stability compared to the raw material of salidroside.SAL-LIPS greatly improved the viability of ARPE-19 cells induced by high glucose of 50～100 µmol·L-1.Compared with the blank control group of ARPE-19 cells,the high glucose group obviously increased the levels of intracellular ROS and MDA,along with enhanced expression of VEGF and HIF-1α.Compared with the high glucose group of ARPE-19 cells,both the low and high concentration SAL-LIPS groups much reduced the levels of intracellular ROS and MDA,and also decreased the expression of VEGF and HIF-1α.Conclusion The constructed SAL-LIPS exhibit good sustained-release effect and stability,which can improve oxidative stress induced in ARPE-19 cells by high glucose,exerting antioxidative effect.
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