Key antimicrobial components of Sanye tablets against Staphylococcus aureus and Escherichia coli
Objective To determine the key components of Sanye tablets(SYT)that exert antimicrobial effect on Staphylococcus aureus(S.aureus)and Escherichia coli(E.coli).Methods The regulatory effects on the gut microbiota of SYT and its antimicrobial activity against S.aureus and E.coli were evaluated in high-fat diet mice model by 16S rRNA sequencing.The antimicrobial activity of total formula,different component-mixtures,and single component of SYT against S.aureus and E.coli was investigated by oxford cup method in vitro to determine the key antimicrobial components in SYT.The minimum inhibitory concentration(MIC)of SYT and its key components against these two pathogenic strains were determined with agar dilution method.Results Sequencing results showed that SYT improved the species richness and diversity,and reduced the abundance of pathogenic bacteria,including S.aureus and E.coli in the gut microbiota of high-fat diet mice.The in vitro antimicrobial assay indicated that the SYT,Salviae Miltiorrhizae Radix et Rhizoma(SMR)-Radix Paeoniae Rubra(RPR)co-components,as well as the respective component of SMR and RPR,had significant inhibitory effect on these two pathogenic bacteria.The MIC of SYT aganist S.aureus and E.coli were 0.781 25 and 3.125 mg·mL-1,respectively.The combination of SMR and RPR showed MIC of 0.25 mg·mL-1 against S.aureus and 3.125 mg·mL-1 against E.coli.The SMR showed MIC of 0.125 mg·mL-1 against S.aureus.The MIC of the RPR was 3.125 mg·mL-1 against E.coli,lower than that of other single components.Conclusion SYT shows inhibitory effect on the common intestinal pathogens such as S.aureus and E.coli.SMR is the key component of SYT against S.aureus,and RPR is the key component against E.coli.
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