Quality markers for Astragalus membranaceus in tonifying qi and lifting yang based on fingerprint and network pharmacology
Objective To determine quality markers for Astragalus membranaceus tonifying qi and lifting yang based on HPLC-ELSD fingerprint network pharmacology and molecular docking.Methods Pharmacodynamic experiment was conducted.HPLC-ELSD was used to establish fingerprints of different Astragalus membranaceus samples and obtain the complete in Astragalus membranaceus chemical compositions information.With network pharmacology,the chemical targets and related pathways of Astragalus membranaceus were selected from various databases for analysis and established the protein interaction network and the composition-target-pathway-disease graph.Molecular docking was used to verify the quality markers and core targets,and to predict the components of Astragalus membranaceus in tonifying qi and lifting yang.Results Pharmacodynamic experiments showed that the decoction of Astragalus membranaceus down-regulated the levels of malondialdehyde,lactic acid accumulation,creatine kinase activity,and tumor necrosis factor-α,and up-regulated the levels of adenosine triphosphate,adenosine diphosphate,splenic index,and thymus index to tonifying qi and lifting yang,delay fatigue,and exert anti-inflammatory and anti-tumour effects.The fingerprint of Astragalus membranaceus identified 10 common peaks.Together with the network pharmacology,the candidate chemical components were analysed and screened to obtain 14 core targets and 20 metabolic pathways.The molecular docking showed that the predicted quality markers bound well to the target molecules.Conclusion Based on measurability and validity of quality markers,combining pharmacodynamic experiments,fingerprinting,network pharmacology,and molecular docking,we related the traditional efficacy of Astragalus membranaceus to modern diseases,and preliminarily predict that astragaloside,mauritian isoflavone glucoside,astragaloside Ⅱ,astragaloside Ⅲ,and isoastragaloside Ⅰ,are the five core chemical components of Astragaloside membranaceus.They tonifying qi and lifting yang through the modulation of multiple target sites and multiple pathways.
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