Objective To determine the possible application of Xinlikang capsules for hematological tumors.Methods MTS assay was used to detect the inhibitory effect of Xinlikang capsule on the proliferation of 8 strains of human hematological tumor cells.Flow cytometry was used to detect the effect of Xinlikang capsules on the cell cycle arrest and apoptosis promotion of acute myeloid leukemia MV-4-11 cells.The protein levels of pro-Caspase 3,cleaved-Caspase 3 and NF-κB p65 in MV-4-11 cells were detected by Western blot.Based on the mice loaded with MV-4-11 subcutaneous tumor transplantation,the effect of Xinlikang capsules on the tumor growth was studied.Results The IC50 values of Xinlikang capsules on MV-4-11,RS4;11,MM.1S,RPMI-8226,Mino,Jeko-1,OCI-LY10 and TMD8 were(54.05±6.33),(125.33±2.46),(655.87±7.95),(153.33±8.50),(188.70±9.11),(180.87±7.31),(186.10±6.55)and(253.53±8.88)µg·mL-1.Xinlikang capsules promoted the apoptosis of MV-4-11 cells in a concentration-dependent manner.When the concentration reached 500 µg·mL-1,the apoptosis rate was(99.41±0.36)%(P＜0.001),but the effect on cell cycle was weak.Xinlikang capsules promoted the cleavage of pro-Caspase 3 and down-regulate NF-κB p65 in MV-4-11 cells in a concentration-dependent manner.The clinical drug concentration(1000 mg·kg-1)of Xinlikang capsules significantly inhibited the tumor growth of mice loaded with MV-4-11 subcutaneous tumor transplantation,with the inhibition rate of 57.28%.Conclusion Xinlikang capsules can inhibit the proliferation of 8 hematological tumor cell lines.The proliferation inhibition on human acute myeloid leukemia MV-4-11 cells mainly caused by inhibiting the NF-κB pathway and promoting the cell apoptosis.The clinical concentration of Xinlikang capsules may well inhibit the tumor growth of mice loaded with MV-4-11 subcutaneous tumor transplantation,suggesting Xinlikang capsules may be used as an adjuvant therapy for hematologic malignancies.
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