Madecassoside alleviates interstitial cystitis by inhibiting the pyroptosis of NF-κB/NLRP3 pathway
Objective To determine the potential effect of madecassoside on interstitial cystitis(IC)through the pyroptosis mediated by NF-κB/NLRP3.Methods An in vitro IC model induced by lipopolysaccharide in SV-HUC-1 cells was used to evaluate the effect of different concentrations of madecassoside.The protective effects of madecassoside on human ureteral epithelial cells were assessed with the MTT cell viability assay and single-layer cell scratch assay.The inhibitory effects of madecassoside on NF-κB/NLRP3-mediated pyroptosis were investigated through Western blot and real-time PCR analysis.Results Madecassoside enhanced the cell viability and wound healing compared to the model group in vitro.It effectively suppressed the expression of pyroptosis-related proteins and mRNA mediated by the NF-κB/NLRP3 pathways in the cell model.Conclusion Madecassoside demonstrates a protective role in the lipopolysaccharide induced SV-HUC-1 cell model of IC by inhibiting pyroptosis through the NF-κB/NLRP3 pathways.
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