Mechanism of Qiwei Shendi granules for chronic glomerulonephritis through ferroptosis pathway based on network pharmacology
Objective To determine the effectiveness and mechanism of Qiwei Shendi granules in the treatment of chronic glomerulonephritis(CGN)via ferroptosis based on network pharmacology.Methods GeneCards,OMIM,DrugBank,TCMSP,HERB,BATMAN-TCM and FerrDb databases were screened to obtain CGN-related disease targets,active ingredients and targets of Qiwei Shendi granules and ferroptosis-related targets.Cytoscape software was used to construct network of protein-protein interaction(PPI)and active component-action target.GO enrichment and KEGG pathways were conducted with R language.Molecular docking was performed with AutoDockTools 1.5.7.The expression of PPARA,MAPK14,HRAS and PTGS2 were determined in CGN-mice kidney tissues by Westem blot.Results Totally 1896 CGN-related disease targets,564 ferroptosis targets,33 compounds and 405 targets from Qiwei Shendi granules were screened.Totally 17 intersected targets were obtained,which could be potentially therapeutic targets.Four core targets(PPARA,MAPK14,HRAS and PTGS2)in the network were screened with MCODE,Degree,MCC,DMNC,MNC,EPC,EcCentricity,Closeness,Radiality,and BottleNeck algorithms.All docking results showed that the key ingredients in Qiwei Shendi granules had strong binding activity with the core targets.In addition,the four core protein expression changes were reversed in CGN-mice after the treatment with Qiwei Shendi granules.Conclusion Qiwei Shendi granules in regulating CGN via ferroptosis may be related to PPARA,MAPK14,HRAS and PTGS2.
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