Content of 3 components of volatile oil from different parts of Cinnamomum cassia and its in vitro transdermal permeation
Objective To establish a method to simultaneously determine the content of cinnamic acid,cinnamaldehyde and 2-methoxy cinnamaldehyde by HPLC-RID,and to compare the in vitro transdermal properties of volatile oil from different parts of Cinnamomum cassia.Methods Mouse skin was used as the permeation medium,the in vitro transdermal absorption experiments of cinnamon bark oil,cassia twig oil and cinnamon leaf oil were conducted with Franz diffusion cells.We simultaneously determinated the content of cinnamic acid,cinnamaldehyde and 2-methoxy cinnamaldehyde in transdermal samples by Agilent C18 column(4.6 mm×250 mm,5 μm).Acetonitrile-0.2％phosphoric acid(30:70)was used as the mobile phase,and the flow rate were all at 0.9 mL·min-1.The refractive index detector(RID)was used,and the temperatures of column and detector were all at 30 ℃.The accumulative permeation and rate of each component were calculated and analyzed.Results Cinnamic acid,cinnamaldehyde and 2-methoxy cinnamaldehyde were linear within 0.0489～0.5381 μg,0.1816～9.9874 μg,and 0.1142～1.2566 μg,respectively(r＞0.999).The 24 h accumulative permeation per unit area was 53.84,1228.10,and 265.29 μg·cm-2 from the cinnamon bark oil;84.73,2937.69,and 87.39 μg·cm-2 from the cassia twig oil;while 67.45,1699.78,and 347.99 μg·cm-2 from the cinnamon leaf oil.The steady-state permeation rates were 1.41,96.24,and 12.77 μg·cm-2·h-1 from the cinnamon bark oil;1.97,130.01,and 4.90 μg·cm-2·h-1 from the cassia twig oil;and 0.91,74.85,and 16.48 μg·cm-2·h-1 from the cinnamon leaf oil.Conclusion The established HPLC-RID method is fast,simple,with good separation and high accuracy,which can be used for the quality control and transdermal testing of cinnamon oil.All these indicator components in these volatile oils can permeate the mice skin in vitro.The accumulative permeation and rate from largest to smallest are cinnamaldehyde,2-methoxy cinnamaldehyde and cinnamic acid.
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