Optimization of ultrasound-assisted extraction of plumbagin by response surface methodology
Objective To optimize the ultrasound-assisted extraction of plumbagin from Plumbago zeylanica L..Methods Ultraviolet spectrophotometry was used to analyze the content of plumbagin.The effects of ultrasound time,extraction temperature,and material-liquid ratio on the yield of plumbagin were analyzed with a single-factor test.The best extraction process was subsequently optimized with the Box-Behnken response surface methodology,based on single-factor tests.Results The adjusted coefficient of determination(RAdj2)of the model was 0.8394,and the probability value(P＜0.005)of the regression model indicated much significance.The optimal conditions for ultrasound-assisted extraction included ultrasound time of 41 min,the extraction temperature at 42 ℃,and the material-liquid ratio at 1∶23(g/mL).Under the optimal conditions,five tests were conducted to verify the results.The average yield of plumbagin was 1.8941％,which was essentially consistent with the predicted value of 1.823％.Conclusion The extraction of plumbagin from Plumbago zeylanica L.using ultrasound-assisted extraction is stable and reliable.
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