响应面法优化兰雪醌超声辅助提取工艺的研究
Optimization of ultrasound-assisted extraction of plumbagin by response surface methodology
莫秋月 1时晨 1袁慧 1李春霜 1姚泽 1卢明 1罗紫月 2别松涛3
作者信息
- 1. 天津中医药大学中药制药工程学院,天津 301617
- 2. 天津中医药大学中医学院,天津 301617
- 3. 天津中医药大学中药制药工程学院,天津 301617;天津中医药大学组分中药国家重点实验室,天津 301617;现代中医药海河实验室,天津 301617
- 折叠
摘要
目的 优化白花丹中兰雪醌超声辅助提取的最佳工艺.方法 采用紫外分光光度法分析兰雪醌含量.采用单因素试验考察超声时间、提取温度和料液比对兰雪醌得率的影响,并在单因素试验的基础上使用Box-Behnken响应面法优化得到最佳工艺.结果 该模型的调整决定系数(RAdj2)为0.8394,回归模型的概率值(P＜0.005)显示出较高的显著性.通过Box-Behnke 响应面法得到超声提取兰雪醌的最佳工艺参数为超声时间 41 min,提取温度42℃,料液比1∶23(g/mL),预测兰雪醌得率为1.823％.在最佳条件下进行了 5次验证试验,得到兰雪醌平均得率为1.8941％,与预测值基本吻合.结论 利用超声辅助提取方法提取白花丹中兰雪醌的工艺稳定可靠.
Abstract
Objective To optimize the ultrasound-assisted extraction of plumbagin from Plumbago zeylanica L..Methods Ultraviolet spectrophotometry was used to analyze the content of plumbagin.The effects of ultrasound time,extraction temperature,and material-liquid ratio on the yield of plumbagin were analyzed with a single-factor test.The best extraction process was subsequently optimized with the Box-Behnken response surface methodology,based on single-factor tests.Results The adjusted coefficient of determination(RAdj2)of the model was 0.8394,and the probability value(P＜0.005)of the regression model indicated much significance.The optimal conditions for ultrasound-assisted extraction included ultrasound time of 41 min,the extraction temperature at 42 ℃,and the material-liquid ratio at 1∶23(g/mL).Under the optimal conditions,five tests were conducted to verify the results.The average yield of plumbagin was 1.8941％,which was essentially consistent with the predicted value of 1.823％.Conclusion The extraction of plumbagin from Plumbago zeylanica L.using ultrasound-assisted extraction is stable and reliable.
关键词
白花丹/兰雪醌/超声辅助提取/响应面优化Key words
Plumbago zeylanica L./plumbagin/ultrasound-assisted extraction/response surface methodology引用本文复制引用
基金项目
天津市科技计划项目(22ZYJDSS00100)
现代中医药海河实验室科技项目(22HHZYSS00003)
出版年
2024
NETL
NSTL
万方数据