Different parts of Vinegar-baked Bupleuri Radix water extract affect the distribution of rhein and hepatic nuclear factor 1/4αby single administration
Objective To determine the distribution of different parts of Vinegar-baked Bupleuri Radix(VBRB)on rhein in rats and its effect on liver nuclear factor 1α and 4α and to preliminarily explore the active sites and regulatory links of the liver targeted enhancement effect of VBRB.Methods Rhein was orally administered in rats in combination with polysaccharides(PSS),n-butanol(BUF),and small molecule fraction(MHE)of VBRB.The blood and tissues were collected from the abdominal aorta at 5,15,30,90,and 210 min after the administration.The contents of rhein and phase Ⅱ metabolic enzymes in the plasma and the tissues were measured,and the relative targeting rate(RTE)and relative uptake rate(Re)were calculated to evaluate the effects of different parts of VBRB on rhein tissue targeting.Results Based on the RTE and Re,all three parts of VBRB increased the liver targeting of rhein in the order of MHE＞PSS＞BUF.After the administration of rhein for 30 min,PSS and BUF decreased the activity of SULT1A,while HNF1α/4α content was increased by MHE.Conclusion All three parts are the active parts of VBRB,with different parts aiming different targets,and the mechanism needs verification.
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