Content determination of active ingredients in Jinlianhua soft capsules by HPLC
Objective To simultaneously determine the content of orientin and vitexin in Jinlianhua soft capsules by HPLC.Methods An RD-C18(4.6 mm ×250 mm,5 μm)chromatographic column was used with a gradient elution of acetonitrile and 0.1％phosphoric acid solution as the mobile phase.The flow rate of 1.0 mL·min-1,the wavelength was 340 nm,with the column temperature of 40 ℃,and the injection volume of 10 μL.Results The linear range of orientin and vitexin was 1.94～194.8μg·mL-1(r=1.000)and 1.03～103.7 μg·mL-1(r=1.000).The average recovery was 103.6％and 100.2％,and RSD was 0.64％and 0.78％(n=6).The contents of orientin and vitexin in the 14 batches ranged 3.090～4.729 mg·g-1 and 1.104～1.402 mg·g-1.Conclusion The method is simple and accuracy,which can be used for the quality control of Jinlianhua soft capsules.
Jinlianhua soft capsuleHPLCorientinvitexinquality standard
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