Protective effect of salvia miltiorrhiza-panax notoginseng against microglia cell injury induced by oxygen-glucose deprivation/reperfusion
Objective To determine the protective effect of salvia miltiorrhiza-panax notoginseng(SM-PN)with different proportions against BV2 cell injury induced by oxygen-glucose deprivation/reperfusion(OGD/R),and its possible mechanism.Methods BV2 cells were cultured in vitro and the models were established by OGD/R.The cells were randomly divided into a blank group,a model group,and an SM-PN+OGD/R group.The survival rate of cells in each group was screened by CCK-8,and the best SM-PN matching group was selected for subsequent experiments.The expression of TNF-α,IL-1β,IL-6,iNOS,TGF-β and IL-10 mRNA was analyzed by Real-time PCR.The protein levels of IκBα,p-IκBα,p65 and p-p65 were determined by Western blot.Results Compared with the model group,the cell viability of SM-PN 5∶3 groups at 200 mg·L-1 significantly increased,IκBα and p65 protein phosphorylation levels markedly decreased,the mRNA expresion level of iNOS,TNF-α,IL-1β,and IL-6 was significantly lower(P＜0.05),and that of TGF-β and IL-10 was significantly higher(P＜0.05).Conclusion SM-PN can inhibit OGD/R-induced neuroin flammatory responses in BV2 cells,whose mechanism may be related to the inhibition of NF-κB pathway,promoting the polarization of the microglia from M1 to M2 phenotype.
herbal pair of salvia miltiorrhiza-panax notoginsengmicroglia celloxygen-glucose deprivation/reperfusioninflammation
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