Chemical composition and blood compontents of the water solution of ultrafine granular powder of Erchen decoction modified formula
Objective To determine the chemical compositions and blood compontents in the water solution of ultrafine granular powder of Erchen decoction modified formula.Methods Ultra performance liquid chromatography quadrupole electrostatic field Orbitrap high resolution mass spectrometry(UHPLC-Q-Exactive-MS)was used to compare the chromatograms of compound samples,blank serum and drug containing serum,and analyze the prototype and metabolites of the water solution of ultrafine granular powder of Erchen decoction modified formula in the rat serum.Results Totally 151 chemical components were identified from the water solution of ultrafine granular powder of Erchen decoction modified formula.After comparison with standard substance,11 components(citric acid,chlorogenic acid,hesperidin,poric acid B,ursolic acid,gastrodin,parishin A,vitexin,hyperoside,hinsenoside Rg1,and ginsenoside Rd)were ultimately determined.Totally 33 blood components were identified,28 of which were prototypes and 5 were metabolites.Conclusion The blood components in the water solution of ultrafine granular powder of Erchen decoction modified formula are determined by UHPLC-Q-Exactive-MS,providing a reference for further elucidating the pharmacological substance basis and mechanism of its action.
Erchen decoctionultrafine granular powder of Chinese materia medicamodified formulablood compontentUHPLC-Q-Exactive-MS
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