Preparation of PLGA-PEG-PLGA thermal gel for wound analgesics and healing
Objective To prepare a temperature-sensitive gel system composed of PLGA-PEG-PLGA/glycerol for loading bupivacaine hydrochloride and polydeoxyribonucleotide combination drugs.Methods Gelling temperature was used as the key response parameter,the gel matrix concentration,drug loading and glycerol addition were preliminarily screened by a single factor method.Subsequently,an orthogonal experiment was designed,and osmotic pressure was introduced as a parameter to optimize the formulation.Finally,the quality of the selected formulations was evaluated.Results The preferred formula contained 16％PLGA-PEG-PLGA,5％glycerol,2％bupivacaine hydrochloride,and 0.4％polydeoxyribonucleotide.The formula was a uniform transparent solution at room temperature,with gelling temperature of 33.83 ℃,gelling time of 100 s,pH value of 3.11,and osmotic pressure of 836 mOsm,with good injectability and adhesion.The drug release conformed to the Ritger-peppas equation.The open wound model confirmed that the sample effectively promoted wound healing and showed analgesic effect.Conclusion The preparation of the optimal formula is simple,and all quality indicators meet the requirements,which is expected to be used in clinical practice.
temperature-sensitive in situ gelprescription screeninganalgesiahealing promotionbupivacaine hydrochloridepolydeoxyribonucleotide
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