Improvement mechanism of Jianwei Xiaoshi tablets on infantile anorexia based on proteomics
Objective To determine the improvement effect and mechanism of Jianwei Xiaoshi tablets on infantile anorexia by proteomics.Methods 3-week-old SD rats were randomly divided into 5 groups after 4 weeks of high-fat and high-protein diet modeling:a control group,a model group,a domperidone 10 mg·kg-1 group,a Jianwei Xiaoshi tablets 2 g·kg-1 group and a Jianwei Xiaoshi tablets 5 g·kg-1 group.After one-week administration,the body weight and gastrointestinal motility indicators of the rats were measured,and the blood and gastric tissues were collected for plasma hormone levels and proteomics analysis,respectively.Results Oral administration of Jianwei Xiaoshi tablets significantly increased the food intake and gastrointestinal motility in rats with infantile anorexia.While the plasma motilin(MTL)and β-endorphin(β-EP)increased,the level of somatostatin(SST)decreased.Proteomic analysis revealed that the pathogenesis of infantile anorexia model rats,as well as the therapeutic process of Jianwei Xiaoshi tablets,was associated with differential expression of several proteins.Among these proteins,trypsinogen 2(Prss2)played a key role in digestion and protein absorption.Additionally,Ndufb2 was involved in endogenous cannabinoid signaling,facilitating ingestion in the activated state.During the model induction,the expression of both proteins was down-regulated,but after gavage with Jianwei Xiaoshi tablets,their expression was up-regulated.Furthermore,several signaling pathways might involved in the treatment with Jianwei Xiaoshi tablets.Notably,the regulatory pathway of actin cytoskeleton was obviously enriched in the gastric tissues after the treatment.This pathway promoted the contraction of the gastrointestinal smooth muscle.Conclusion Jianwei Xiaoshi tablets may improve infantile anorexia by increasing food intake and gastrointestinal motility,regulating the secretion of MTL,β-EP,and SST in infantile anorexic rats.Prss2 and Ndufb2 proteins,along with the actin cytoskeleton regulatory pathway,not only are involved in the pathogenesis of infantile anorexia but also play a role in the treatment of infantile anorexia with Jianwei Xiaoshi tablets.These proteins and pathways may serve as important targets of the therapeutic effect of Jianwei Xiaoshi tablets.
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