Simulated gastrointestinal digestion in vitro of Coix seed glutelin and the optimization of hydrolysis conditions
Objective To determine the effect of simulated gastrointestinal digestion in vitro on the activity of angiotensin-converting enzyme(ACE)inhibitory peptides from Coix seed glutelin,and to optimize the hydrolysis conditions.Methods Coix seed glutelin was hydrolyzed under simulated gastrointestinal contiditions.The components were separated by ultrafiltration with 3 kDa molecular weight cut off,and the ACE inhibition rate was determined by RP-HPLC method.Antihypertensive efficacy of the potently active components were further evaluated in spontaneously hypertensive rats.The hydrolysis conditions were finally optimized by orthogonal experiment with hydrolysis degree as the index.Results At the concentration of 0.04 mg·mL-1,the ACE inhibition activity of≤3 kDa component from the simulated intestinal digestion by trypsin ranked first at(99.94±0.01)％.Single administration of this hydrolysate significantly reduced the blood pressure in spontaneously hypertensive rats.The optimum conditions for trypsin hydrolysis of Coix seed glutelin were as follows:hydrolysis time of 6 h,substrate concentration 1.5％,enzyme-substrate ratio at 1:5,and the corresponding degree of hydrolysis was(16.37±1.68)％.Conclusion Coix seed glutelin can be used to generate small molecular peptides with obvious antihypertension effect after simulated gastrointestinal digestion,which can further help develop antihypertension drugs or functional foods.
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