响应面法优化涡旋辅助提取胆木中异长春花苷内酰胺的工艺
Optimization of vortex-assisted extraction of strictosamide from Nauclea officinalis by response surface method
樊小红 1吕菲菲 1赵祥升 1魏建和2
作者信息
- 1. 中国医学科学院北京协和医学院药用植物研究所海南分所,海南省南药资源保护与开发重点实验室,海口 570311
- 2. 中国医学科学院北京协和医学院药用植物研究所海南分所,海南省南药资源保护与开发重点实验室,海口 570311;中国医学科学院北京协和医学院药用植物研究所,中草药物质基础与资源利用教育部重点实验室,濒危药材繁育国家工程实验室,北京 100193
- 折叠
摘要
目的 采用响应面法优化胆木中异长春花苷内酰胺的涡旋辅助提取工艺.方法 在单因素试验的基础上通过Box-Behnken方法对乙醇浓度、涡旋转速、液料比等参数进行优化,建立参数与响应值之间的数学模型,确定最佳提取工艺.结果 生物碱的最佳工艺为:乙醇浓度55％,涡旋转速1500 r·min-1,液料比60 mL/g,涡旋时间1 min,该条件下异长春花苷内酰胺的实际提取率为3.85 mg·g-1,与理论模拟值(3.82mg·g-1)接近.结论 该提取方法简便、快速、提取率高,适用于胆木生物碱的提取,研究结果为胆木的产业化加工提供了理论基础.
Abstract
Objective To optimize the vortex-assisted extraction of strictosamide from Nauclea offiicinalis with the response surface method.Methods The extraction parameters such as ethanol concentration,vortex rotation,and liquid-solid ratio were optimized through Box-Behnken design based on single-factor experiments.The mathematical model between the parameters and the response values was established to determine the best vortex extraction.Results The optimal conditions included ethanol concentration at 55％,vortex rotation at 1500 r·min-1,liquid-solid ratio of 60 mL/g,and vortex time for 1 min.Under these conditions,the yield of strictosamide was 3.85 mg·g-1,close to the theoretical value of 3.82 mg·g-1.Conclusion The extraction is simple,fast and efficient,which is suitable for extracting alkaloids from Nauclea officinalis.It provides a theoretical basis for the industrial process of Nauclea officinalis.
关键词
胆木/异长春花苷内酰胺/涡旋/响应面分析/提取工艺Key words
Nauclea officinalis/strictosamide/vortex/response surface analysis/extraction引用本文复制引用
出版年
2024
NETL
NSTL
万方数据