Potential pharmacodynamic components and mechanism of Shenmu Anshen capsules in improving insomnia based on UPLC-MS/MS combined with network pharmacology
Objective To determine the potential pharmacodynamic components and mechanism of Shenmu Anshen capsules in improving insomnia.Methods The main chemical components of Shenmu Anshen capsules were identified by UPLC-QE-Orbitrap-MS information and literature data.Through TCMSP database and literature review,the potential active components of Shenmu Anshen capsule were screened and supplemented.The potential targets of active components were predicted by Swiss Target Prediction database.OMIM,GeneCards,and TTD databases were used to collect insomnia-related targets.Venny software was used to collect the intersection of components targets and drug targets.Cytoscape software was used to establish the interaction network of drug-compound-target.The STRING platform was used to establish the PPI network.Functional enrichment analysis of target GO and KEGG pathways conducted with the Metascape database,and the component-target-pathway diagram was established by Cytoscape.At last,the mouse model of insomnia caused by p-chlorophenylalanine was used to determine the effect of Shenmu Anshen capsules in improving sleep in the mouse model of insomnia.The serum level of 5-hydroxytryptamine(5-HT)was detected by ELISA.Results Through comparison with the database,literature analysis and mass spectrometry cleavage law analysis,40 compounds were identified from the samples of Shenmu Anshen capsules.Totally of 122 candidate components and 917 predicted targets were screened by network pharmacology.There were 185 common targets with insomnia.The key targets mainly involved TP53,EP300,HDAC1,TNF,APP,etc.Shenmu Anshen capsules mainly participated in the regulation of signaling pathways of neurodegeneration-multiple diseases,dopaminergic synapse,Parkinson disease and serotonergic synapses.Compared with the model group,Shenmu Anshen capsules at medium and high doses greatly prolonged sleep duration and increased the content of 5-HT in the serum.Conclusion The chemical components of Shenmu Anshen capsules and the mechanism of improving neurasthenia insomnia are primarily clarified.Shenmu Anshen capsules can improve the sleep of mice by activating inhibitory monoamine neurotransmitters in the brain and increasing the control of the sleep center by the central nervous system.
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