Transdermal permeability and anti-aging skin effects of copper glycyl-histidine-tripeptide in facial masks
Objective To determine the transdermal permeability of copper glycyl-histidine-tripeptide in facial masks and their effect on skin aging.Methods In vitro mouse skin was used as the model material,and Franz diffusion cell method was used to measure the resident amount in the dermis and cumulative transdermal permeation of copper glycyl-histidine-tripeptide in both generation Ⅰ and Ⅱ masks.Additionally,a mouse model of skin aging was induced by continuous D-galactose administration combined with UV radiation for 60 days.The water content,hyaluronic acid,type Ⅰ collagen content in mouse skin,and expression levels of MMP-1 and MMP-2 in the plasma were measured to determine the effect of copper glycyl-histidine-tripeptide in facial masks on skin aging.Results After 20 minutes application,the resident amount in the mouse dermis and cumulative transdermal permeation of copper glycyl-histidine-tripeptide were approximately 5.8 and 4.6 times higher in the generation n mask as compared with those of generation Ⅰ mask.Compared to generation Ⅰ mask,the generation n mask greatly increased the water content,hyaluronic acid level,and type Ⅰ collagen content in the aging skin of mice,while much reduced the expression levels of MMP-1 and MMP-2 in the mouse plasma.Conclusion Generation n masks containing copper glycyl-histidine-tripeptide show better skin permeability against aging skin in mice.
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